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Substrate binding in vitro and kinetics of RsrI [N6-adenine] DNA methyltransferase

RsrI [N6-adenine] DNA methyltransferase (M·RsrI), which recognizes GAATTC and is a member of a restriction–modification system in Rhodobacter sphaeroides, was purified to >95% homogeneity using a simplified procedure involving two ion exchange chromatographic steps. Electrophoretic gel retardatio...

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Bibliographic Details
Main Authors: Szegedi, Sandra S., Reich, Norbert O., Gumport, Richard I.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2000
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC110777/
https://ncbi.nlm.nih.gov/pubmed/11024176
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