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Comparing protocols for preparation of DNA-free total yeast RNA suitable for RT-PCR
BACKGROUND: Preparation of RNA free from DNA is a critical step before performing RT-PCR assay. Total RNA isolated from several sources, including those obtained from Saccharomyces cerevisiae, using routine methodologies are frequently contaminated with DNA, which can give rise to amplification prod...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2005
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1097730/ https://ncbi.nlm.nih.gov/pubmed/15833107 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-6-9 |
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