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Comparing protocols for preparation of DNA-free total yeast RNA suitable for RT-PCR

BACKGROUND: Preparation of RNA free from DNA is a critical step before performing RT-PCR assay. Total RNA isolated from several sources, including those obtained from Saccharomyces cerevisiae, using routine methodologies are frequently contaminated with DNA, which can give rise to amplification prod...

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Bibliografische gegevens
Hoofdauteurs: Del Aguila, Eduardo M, Dutra, Marcio B, Silva, Joab T, Paschoalin, Vânia MF
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: BioMed Central 2005
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1097730/
https://ncbi.nlm.nih.gov/pubmed/15833107
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-6-9
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