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Isolation of Intact Plastids from Protoplasts from Castor Bean Endosperm

Protoplasts were prepared from castor bean (Ricinus communis) endosperm by treatment with a mixture of the commercial enzymes Macerozyme R-10 and Cellulose “Onozuka” R-10. The protoplasts were gently ruptured by forcing the suspension through a hypodermic needle and the homogenate centrifuged on a l...

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Bibliographic Details
Main Authors: Nishimura, Mikio, Beevers, Harry
Format: Artigo
Language:Inglês
Published: 1978
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1092051/
https://ncbi.nlm.nih.gov/pubmed/16660465
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