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Increased DNA microarray hybridization specificity using sscDNA targets
BACKGROUND: The most widely used amplification method for microarray analysis of gene expression uses T7 RNA polymerase-driven in vitro transcription (IVT) to produce complementary RNA (cRNA) that can be hybridized to arrays. However, multiple rounds of amplification are required when assaying very...
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Main Authors: | , , , , , |
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格式: | Artigo |
語言: | Inglês |
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BioMed Central
2005
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在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1090574/ https://ncbi.nlm.nih.gov/pubmed/15847692 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-6-57 |
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