Increased DNA microarray hybridization specificity using sscDNA targets

BACKGROUND: The most widely used amplification method for microarray analysis of gene expression uses T7 RNA polymerase-driven in vitro transcription (IVT) to produce complementary RNA (cRNA) that can be hybridized to arrays. However, multiple rounds of amplification are required when assaying very...

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Detalhes bibliográficos
Main Authors: Barker, Christopher S, Griffin, Chandi, Dolganov, Gregory M, Hanspers, Kristina, Yang, Jean Yee Hwa, Erle, David J
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2005
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1090574/
https://ncbi.nlm.nih.gov/pubmed/15847692
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-6-57
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