Nested PCR-Denaturing Gradient Gel Electrophoresis Approach To Determine the Diversity of Sulfate-Reducing Bacteria in Complex Microbial Communities

Here, we describe a three-step nested-PCR-denaturing gradient gel electrophoresis (DGGE) strategy to detect sulfate-reducing bacteria (SRB) in complex microbial communities from industrial bioreactors. In the first step, the nearly complete 16S rRNA gene was amplified using bacterial primers. Subseq...

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Main Authors: Dar, Shabir A., Kuenen, J. Gijs, Muyzer, Gerard
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2005
Teme:
Methods
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1087575/
https://ncbi.nlm.nih.gov/pubmed/15870318
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.5.2325-2330.2005
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