Homologous and Heterologous Overexpression in Clostridium acetobutylicum and Characterization of Purified Clostridial and Algal Fe-Only Hydrogenases with High Specific Activities

Clostridium acetobutylicum ATCC 824 was selected for the homologous overexpression of its Fe-only hydrogenase and for the heterologous expressions of the Chlamydomonas reinhardtii and Scenedesmus obliquus HydA1 Fe-only hydrogenases. The three Strep tag II-tagged Fe-only hydrogenases were isolated wi...

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Bibliographic Details
Main Authors: Girbal, Laurence, von Abendroth, Gregory, Winkler, Martin, Benton, Paul M. C., Meynial-Salles, Isabelle, Croux, Christian, Peters, John W., Happe, Thomas, Soucaille, Philippe
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2005
Subjects:
Enzymology and Protein Engineering
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1087525/
https://ncbi.nlm.nih.gov/pubmed/15870373
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.5.2777-2781.2005
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