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Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR

Although PCR and RT-PCR provided a valuable approach for detection of pathogens, the high level of sensitivity of these assays also makes them prone to false positive results. In addition to cross-contamination with true positive samples, false positive results are also possible due to "carry-o...

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Detalhes bibliográficos
Autor principal: Kleiboeker, Steven B
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1087508/
https://ncbi.nlm.nih.gov/pubmed/15823209
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-2-29
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