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Efficient biallelic mutagenesis with Cre/loxP-mediated inter-chromosomal recombination
The isolation of mutant cells with phenotypes caused by random mutagenesis has been hampered in mammalian cells because there are two alleles per gene and the disruption of both alleles is extremely rare. We describe a method for the efficient biallelic mutagenesis in embryonic stem cells. loxP site...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2002
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1084110/ https://ncbi.nlm.nih.gov/pubmed/11964386 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/embo-reports/kvf097 |
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