Agrobacterium tumefaciens-Mediated Transformation of Aspergillus fumigatus: an Efficient Tool for Insertional Mutagenesis and Targeted Gene Disruption

Agrobacterium tumefaciens was used to transform Aspergillus fumigatus by either random or site-directed integration of transforming DNA (T-DNA). Random mutagenesis via Agrobacterium tumefaciens-mediated transformation (ATMT) was accomplished with T-DNA containing a hygromycin resistance cassette. Co...

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Detalhes bibliográficos
Main Authors: Sugui, Janyce A., Chang, Yun C., Kwon-Chung, K. J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1082565/
https://ncbi.nlm.nih.gov/pubmed/15812003
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.4.1798-1802.2005
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