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Partial Purification, Photoaffinity Labeling, and Properties of Mung Bean UDP-Glucose:Dolicholphosphate Glucosyltransferase

UDP-glucose:dolichylphosphate glucosyltransferase has been purified 734-fold from Triton X-100 solubilized mung bean (Phaseolus aureus) microsomes. The partially purified enzyme has broad pH optima of activity from 6.0 to 7.0 and is maximally stimulated with 10 millimolar MgCl(2). The K(m) for UDP-g...

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Bibliografiske detaljer
Main Authors: Drake, Richard R., Kaushal, Gur P., Pastuszak, Irena, Elbein, Alan D.
Format: Artigo
Sprog:Inglês
Udgivet: 1991
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1081011/
https://ncbi.nlm.nih.gov/pubmed/16668398
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