Spinach Leaf Acetyl-Coenzyme A Synthetase: Purification and Characterization

Acetyl-coenzyme A (CoA) synthetase was purified 364-fold from leaves of spinach (Spinacia oleracea L.) using ammonium sulfate fractionation followed by ion exchange, dye-ligand, and gel permeation chromatography. The final specific activity was 2.77 units per milligram protein. The average M(r) valu...

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Main Authors: Zeiher, Carolyn A., Randall, Douglas D.
Format: Artigo
Jezik:Inglês
Izdano: 1991
Teme:
Metabolism and Enzymology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1080781/
https://ncbi.nlm.nih.gov/pubmed/16668197
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