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Binding of 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole to an Essential Cysteine Residue(s) in the Tonoplast H(+)-ATPase from Mung Bean (Vigna radiata L.) Hypocotyls

Vacuolar-type H(+)-ATPase was solubilized from tonoplasts of mung bean (Vigna radiata L.) and purified on a Mono Q anion-exchange column by fast protein liquid chromatography. The purified enzyme was inactivated by the reactive adenine analog, 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl). This i...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Yamanishi, Hiroyasu, Kasamo, Kunihiro
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 1992
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC1080514/
https://ncbi.nlm.nih.gov/pubmed/16668936
Tagiau: Ychwanegu Tag
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