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Solubilization, Partial Purification, and Immunodetection of Squalene Synthetase from Tobacco Cell Suspension Cultures

Squalene synthetase, an integral membrane protein and the first committed enzyme for sterol biosynthesis, was solubilized and partially purified from tobacco (Nicotiana tabacum) cell suspension cultures. Tobacco microsomes were prepared and the enzyme was solubilized from the lipid bilayer using a t...

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Bibliographic Details
Main Authors: Hanley, Kathieen, Chappell, Joseph
Format: Artigo
Language:Inglês
Published: 1992
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1080171/
https://ncbi.nlm.nih.gov/pubmed/16668616
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