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Transient Gene Expression in Protoplasts of Phaseolus vulgaris Isolated from a Cell Suspension Culture

A cell suspension culture of Phaseolus vulgaris cv Negro jamapa was established. Protoplasts isolated from this culture are suitable for transient gene expression studies after DNA transfer by either electroporation or treatment with polyethylene glycol. The optimized conditions for both methods wer...

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Detalles Bibliográficos
Main Authors: Leon, Patricia, Planckaert, Frederique, Walbot, Virginia
Formato: Artigo
Idioma:Inglês
Publicado: 1991
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1077633/
https://ncbi.nlm.nih.gov/pubmed/16668081
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