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An H(+)-ATPase Assay: Proton Pumping and ATPase Activity Determined Simultaneously in the Same Sample
A continuous spectrophotometric assay of H(+)-ATPase activity was developed by combining two well-known methods for measuring proton pumping and ATPase activity. Proton uptake into plasma membrane vesicles from Avena sativa L. (cv Rhiannon) was monitored as the absorbance decrease at 495 nm of the Δ...
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| Autor principal: | |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1077317/ https://ncbi.nlm.nih.gov/pubmed/16667867 |
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