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Isolation of Chloroplastic Phosphoglycerate Kinase : Kinetics of the Two-Enzyme Phosphoglycerate Kinase/Glyceraldehyde-3-Phosphate Dehydrogenase Couple
We report here a method for the isolation of high specific activity phosphoglycerate kinase (EC 2.7.2.3) from chloroplasts. The enzyme has been purified over 200-fold from pea (Pisum sativum L.) stromal extracts to apparent homogeneity with 23% recovery. Negative cooperativity is observed with the t...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1077223/ https://ncbi.nlm.nih.gov/pubmed/16667700 |
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