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Expression of lacZ from the Promoter of the Escherichia coli spc Operon Cloned into Vectors Carrying the W205 trp-lac Fusion

The expression of lacZ has been analyzed and compared in a series of promoter cloning vectors by measuring the amount of lacZ mRNA by hybridization and the amount of β-galactosidase by standard enzymatic assay. Expression was driven by the promoter, P(spc), of the spc ribosomal protein operon. The v...

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Bibliografiske detaljer
Main Authors: Liang, Sung-Tzu, Dennis, Patrick P., Bremer, Hans
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 1998
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC107692/
https://ncbi.nlm.nih.gov/pubmed/9829916
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