Purification, Gene Cloning, Targeted Knockout, Overexpression, and Biochemical Characterization of the Major Pyrazinamidase from Mycobacterium smegmatis

The pyrazinamidase from Mycobacterium smegmatis was purified to homogeneity to yield a product of approximately 50 kDa. The deduced amino-terminal amino acid sequence of this polypeptide was used to design an oligonucleotide probe for screening a DNA library of M. smegmatis. An open reading frame, d...

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Foilsithe in:	J Bacteriol
Príomhchruthaitheoirí:	Boshoff, Helena I. M., Mizrahi, Valerie
Formáid:	Artigo
Teanga:	Inglês
Foilsithe / Cruthaithe:	American Society for Microbiology (ASM) 1998
Ábhair:	Genetics and Molecular Biology
Rochtain ar líne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC107651/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9811635/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.180.22.5809-5814.1998
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