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NAD(+)-Linked Isocitrate Dehydrogenase: Isolation, Purification, and Characterization of the Protein from Pea Mitochondria

The NAD(+)-dependent isocitrate dehydrogenase from etiolated pea (Pisum sativum L.) mitochondria was purified more than 200-fold by dye-ligand binding on Matrix Gel Blue A and gel filtration on Superose 6. The enzyme was stabilized during purification by the inclusion of 20% glycerol. In crude matri...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: McIntosh, Cecilia A., Oliver, David J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1992
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1075518/
https://ncbi.nlm.nih.gov/pubmed/16653002
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