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Improvement of Longevity and Viability of Sperm Cells Isolated from Pollen of Zea mays L.

Our previous studies showed that the common maize (Zea mays L.) sperm isolation medium (Brewbaker and Kwack salts in 0.44 m sucrose without buffering) caused cell lysis in vitro. In an attempt to remedy this situation, 6 sugars, 10 buffers, 5 pH values, and 3 membrane protective agents were screened...

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Detalhes bibliográficos
Main Authors: Zhang, Guichang, Williams, Connie M., Campenot, Mary K., McGann, Locksley E., Cass, David D.
Formato: Artigo
Idioma:Inglês
Publicado em: 1992
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1075515/
https://ncbi.nlm.nih.gov/pubmed/16652985
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