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Isolation and Characterization of Tonoplast from Chilling-Sensitive Etiolated Seedlings of Vigna radiata L.

Tonoplasts were isolated in a high purity from etiolated young seedlings of Vigna radiata L. (mung bean) utilizing a sucrose density gradient system. The excised hypocotyls were homogenized in a sorbitol-buffer system and the 3,600 to 156,000g pellets obtained after the differential centrifugations...

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Detalhes bibliográficos
Main Authors: Yoshida, Shizuo, Kawata, Takefumi, Uemura, Matsuo, Niki, Teruo
Formato: Artigo
Idioma:Inglês
Publicado em: 1986
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1075075/
https://ncbi.nlm.nih.gov/pubmed/16664574
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