Probing the Role of Cysteine Residues in Glucosamine-1-Phosphate Acetyltransferase Activity of the Bifunctional GlmU Protein from Escherichia coli: Site-Directed Mutagenesis and Characterization of the Mutant Enzymes

The glucosamine-1-phosphate acetyltransferase activity but not the uridyltransferase activity of the bifunctional GlmU enzyme from Escherichia coli was lost when GlmU was stored in the absence of β-mercaptoethanol or incubated with thiol-specific reagents. The enzyme was protected from inactivation...

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Bibliografiske detaljer

Udgivet i:	J Bacteriol
Principais autores:	Pompeo, Frédérique, van Heijenoort, Jean, Mengin-Lecreulx, Dominique
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1998
Fag:	Enzymes and Proteins
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC107502/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9733680/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.180.18.4799-4803.1998
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