Simplified Fluorescent Multiplex PCR Method for Evaluation of the T-Cell Receptor Vβ-Chain Repertoire

Rationale: evaluation of the T-cell receptor (TCR) Vβ-chain repertoire by PCR-based CDR3 length analysis allows fine resolution of the usage of the TCR Vβ repertoire and is a sensitive tool to monitor changes in the T-cell compartment. A multiplex PCR method employing 24 labeled upstream Vβ primers...

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Autores principales: Fernandes, Sanjit, Chavan, Surendra, Chitnis, Vivek, Kohn, Nina, Pahwa, Savita
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2005
Materias:
Cellular Immunology
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1074378/
https://ncbi.nlm.nih.gov/pubmed/15817753
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CDLI.12.4.477-483.2005
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