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Oligohistidine Tag Mutagenesis of the λ Holin Gene

Holins are a diverse group of small integral membrane proteins elaborated by bacteriophages to lyse bacterial hosts and effect release of progeny phages in a precisely timed manner. Recently, the holin S gene of phage λ was overexpressed and the holin protein was purified to homogeneity by means of...

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Bibliographic Details
Main Authors: Smith, David L., Young, Ry
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 1998
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC107418/
https://ncbi.nlm.nih.gov/pubmed/9696770
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