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Positive Selection for Loss-of-Function tat Mutations Identifies Critical Residues Required for TatA Activity
The Tat system, found in the cytoplasmic membrane of many bacteria, is a general export pathway for folded proteins. Here we describe the development of a method, based on the transport of chloramphenicol acetyltransferase, that allows positive selection of mutants defective in Tat function. We have...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1070390/ https://ncbi.nlm.nih.gov/pubmed/15805540 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.8.2920-2925.2005 |
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