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Kinetic Bias in Estimates of Coastal Picoplankton Community Structure Obtained by Measurements of Small-Subunit rRNA Gene PCR Amplicon Length Heterogeneity

Marine bacterioplankton diversity was examined by quantifying natural length variation in the 5′ domain of small-subunit (SSU) rRNA genes (rDNA) amplified by PCR from a DNA sample from the Oregon coast. This new technique, length heterogeneity analysis by PCR (LH-PCR), determines the relative propor...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Principais autores: Suzuki, Marcelino, Rappé, Michael S., Giovannoni, Stephen J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106679/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9797317/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.11.4522-4529.1998
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