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Isolation of Mesophyll Protoplasts from Mature Leaves of Soybeans

A procedure based on a combined cellulase-Pectolyase Y-23 enzyme digestion and metrizamide-sorbitol gradient purification protocol was developed for isolating mesophyll protoplasts from mature leaves of soybean (Glycine max L. Merr.). Based on chlorophyll content, this procedure results in a 10 to 1...

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Bibliografische gegevens
Hoofdauteur: Lin, Willy
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1983
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1066609/
https://ncbi.nlm.nih.gov/pubmed/16663331
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