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Isolation and Transport Properties of Protoplasts from Cortical Cells of Corn Roots

A procedure was developed for the enzymic isolation of large quantities of protoplasts from the cortex of Zea mays L. WF9 × MO 17 roots. Cortex was separated from the primary root, sectioned, and the cell walls digested for 3.5 hours in 2% (w/v) Cellulysin, 0.1% Pectolyase Y-23, 1 millimolar CaCl(2)...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Gronwald, John W., Leonard, Robert T.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 1982
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC1065893/
https://ncbi.nlm.nih.gov/pubmed/16662685
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