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Isolation and Purification of Intact Peroxisomes from Green Leaf Tissue

Intact peroxisomes were prepared from green leaves of a number of C(3) species, both monocots and dicots. A protoplast extract from which chloroplasts have been removed by a 1-minute 10,000g centrifugation was applied to a step gradient of 5, 15, 30, and 45% Percoll containing 0.5 molar sucrose, 0.1...

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Bibliografske podrobnosti
Main Authors: Schmitt, Mark R., Edwards, Gerald E.
Format: Artigo
Jezik:Inglês
Izdano: 1982
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1065852/
https://ncbi.nlm.nih.gov/pubmed/16662640
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