Development of Procedures for Direct Extraction of Cryptosporidium DNA from Water Concentrates and for Relief of PCR Inhibitors

Extraction of high-quality DNA is a key step in PCR detection of Cryptosporidium and other pathogens in environmental samples. Currently, Cryptosporidium oocysts in water samples have to be purified from water concentrates before DNA is extracted. This study compared the effectiveness of six DNA ext...

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Detalhes bibliográficos
Main Authors: Jiang, Jianlin, Alderisio, Kerri A., Singh, Ajaib, Xiao, Lihua
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1065175/
https://ncbi.nlm.nih.gov/pubmed/15746310
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.3.1135-1141.2005
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