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Estimation of the Relative Abundance of Different Bacteroides and Prevotella Ribotypes in Gut Samples by Restriction Enzyme Profiling of PCR-Amplified 16S rRNA Gene Sequences

We describe an approach for determining the genetic composition of Bacteroides and Prevotella populations in gut contents based on selective amplification of 16S rRNA gene sequences (rDNA) followed by cleavage of the amplified material with restriction enzymes. The relative contributions of differen...

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Autors principals: Wood, Jacqueline, Scott, Karen P., Avguštin, Gorazd, Newbold, C. James, Flint, Harry J.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 1998
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC106512/
https://ncbi.nlm.nih.gov/pubmed/9758785
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