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Characterization of a Novel Zinc-Containing, Lysine-Specific Aminopeptidase from the Hyperthermophilic Archaeon Pyrococcus furiosus

Cell extracts of the proteolytic, hyperthermophilic archaeon Pyrococcus furiosus contain high specific activity (11 U/mg) of lysine aminopeptidase (KAP), as measured by the hydrolysis of l-lysyl-p-nitroanilide (Lys-pNA). The enzyme was purified by multistep chromatography. KAP is a homotetramer (38....

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Autors principals: Story, Sherry V., Shah, Claudia, Jenney, Francis E., Adams, Michael W. W.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2005
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1064047/
https://ncbi.nlm.nih.gov/pubmed/15743956
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.6.2077-2083.2005
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