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Regulating Pilin Expression Reveals a Threshold for S Motility in Myxococcus xanthus
An isopropyl-β-d-thiogalactopyranoside (IPTG)-inducible promoter was constructed in Myxococcus xanthus. The single-copy pilA gene encodes pilin, the monomer unit of M. xanthus type IV pili. To vary the level of pilA expression, we cloned its promoter in front of the lac operator, and a plasmid conta...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology
2005
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1064035/ https://ncbi.nlm.nih.gov/pubmed/15743959 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.6.2105-2112.2005 |
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