Expression of Psychrophilic Genes in Mesophilic Hosts: Assessment of the Folding State of a Recombinant α-Amylase

α-Amylase from the antarctic psychrophile Alteromonas haloplanktis is synthesized at 0 ± 2°C by the wild strain. This heat-labile α-amylase folds correctly when overexpressed in Escherichia coli, providing the culture temperature is sufficiently low to avoid irreversible denaturation. In the describ...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Feller, Georges, Le Bussy, Olivier, Gerday, Charles
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	Enzymology and Protein Engineering
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106386/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9501457/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.3.1163-1165.1998
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