Isolation, Characterization, Molecular Gene Cloning, and Sequencing of a Novel Phytase from Bacillus subtilis

The Bacillus subtilis strain VTT E-68013 was chosen for purification and characterization of its excreted phytase. Purified enzyme had maximal phytase activity at pH 7 and 55°C. Isolated enzyme required calcium for its activity and/or stability and was readily inhibited by EDTA. The enzyme proved to...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Kerovuo, Janne, Lauraeus, Marko, Nurminen, Päivi, Kalkkinen, Nisse, Apajalahti, Juha
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	Enzymology and Protein Engineering
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106281/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9603817/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.6.2079-2085.1998
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