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A Polyethylene Glycol-Mediated Protoplast Transformation System for Production of Fertile Transgenic Rice Plants

We have established an efficient procedure for protoplast transformation and regeneration of fertile transgenic plants of rice (Oryza sativa L.) cultivars Nipponbare and Taipei 309. Protoplasts were mixed with a plant-expressible hygromycin resistance gene and treated with 25% (w/v) polyethylene gly...

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Main Authors: Hayashimoto, Akio, Li, Zhijian, Murai, Norimoto
格式: Artigo
語言:Inglês
出版: 1990
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1062601/
https://ncbi.nlm.nih.gov/pubmed/16667593
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