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Purification by Immunoadsorption and Immunochemical Properties of NADP-Dependent Malic Enzymes from Leaves of C(3), C(4), and Crassulacean Acid Metabolism Plants

NADP:malic enzyme from corn (Zea mays L.) leaves was purified by conventional techniques to apparent homogeneity as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Antibodies raised against this protein in rabbits were purified, coupled covalently to protein A-Sepharose CL-4B, a...

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Bibliographic Details
Main Authors: Fathi, Marion, Schnarrenberger, Claus
Format: Artigo
Language:Inglês
Published: 1990
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1062358/
https://ncbi.nlm.nih.gov/pubmed/16667339
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