New Methods for Extraction and Quantitation of Zeins Reveal a High Content of γ-Zein in Modified opaque-2 Maize

We have developed methods for quantitative extraction and analysis of zeins from maize (Zea mays L.) flour. Extraction involved solubilization of total endosperm proteins in an alkaline buffer containing SDS and 2-mercaptoethanol with subsequent precipitation of nonzein proteins by the addition of e...

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Hlavní autoři: Wallace, John C., Lopes, Mauricio A., Paiva, Edilson, Larkins, Brian A.
Médium: Artigo
Jazyk:Inglês
Vydáno: 1990
Témata:
Molecular Biology and Gene Regulation
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1062269/
https://ncbi.nlm.nih.gov/pubmed/16667246
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