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Cytosolic Phosphofructokinase from Spinach Leaves : I. Purification, Characteristics, and Regulation

Cytosolic ATP-dependent phosphofructokinase (PFK) from spinach leaves (Spinacia oleracea L.) was enriched 2600-fold by (NH(4))(2)SO(4) fractionation, DEAE anion exchange chromatography, Blue Sepharose CL-6B, and ATP agarose type 3-affinity chromatography. The final preparation had a specific activit...

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Detalhes bibliográficos
Main Authors: Häusler, Rainer E., Holtum, Joseph A. M., Latzko, Erwin
Formato: Artigo
Idioma:Inglês
Publicado em: 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1061917/
https://ncbi.nlm.nih.gov/pubmed/16666957
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