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Purification and Characterization of Tonoplast ATPase from Etiolated Mung Bean Seedlings

The tonoplast ATPase from etiolated seedlings of Vigna radiata L. (mung bean) was isolated using a two-step detergent solubilization modified from Mandala and Taiz (S Mandala, L Taiz [1985] Plant Physiol 78: 327-333). After ultracentrifugation on 10 to 28% sucrose gradient, the ATPase showed a 31.6-...

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Detalhes bibliográficos
Main Authors: Wang, May Yun, Lin, Ya Hui, Chou, Wing Ming, Chung, Tsuey Ping, Pan, Rong Long
Formato: Artigo
Idioma:Inglês
Publicado em: 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1061749/
https://ncbi.nlm.nih.gov/pubmed/16666796
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