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An Improved Method for Analysis of Polyamines in Plant Tissue by Precolumn Derivatization with o-Phthalaldehyde and Separation by High Performance Liquid Chromatography

An improved high-performance liquid-chromatographic method was developed for estimation of polyamines in crude plant extracts. Polyamines were derivatized with o-phthalaldehyde and mercaptoethanol (OPT). The fluorescent derivatives were eluted from a C(18) column with the dimethylcyclohexylamine-pho...

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Detalhes bibliográficos
Main Authors: Corbin, James L., Marsh, Barry H., Peters, Gerald A.
Formato: Artigo
Idioma:Inglês
Publicado em: 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1061742/
https://ncbi.nlm.nih.gov/pubmed/16666789
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