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UDP-Glucose: (1,3)-β-Glucan Synthase from Daucus carota L. : Characterization, Photoaffinity Labeling, and Solubilization

The membrane-bound UDP-glucose-β-(1,3)-glucan synthase from Daucus carota L. was characterized and a solubilization procedure was developed. The enzyme exhibited maximal activity in the presence of 0.75 millimolar Ca(2+), 0.5 millimolar EGTA, and 5 millimolar cellobiose at pH 7.5 and 30°C at 1 milli...

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Main Authors: Lawson, Stephen G., Mason, Theresa L., Sabin, Robert D., Sloan, Margaret E., Drake, Richard R., Haley, Boyd E., Wasserman, Bruce P.
格式: Artigo
語言:Inglês
出版: 1989
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1061683/
https://ncbi.nlm.nih.gov/pubmed/16666718
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