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UDP-Glucose: (1,3)-β-Glucan Synthase from Daucus carota L. : Characterization, Photoaffinity Labeling, and Solubilization
The membrane-bound UDP-glucose-β-(1,3)-glucan synthase from Daucus carota L. was characterized and a solubilization procedure was developed. The enzyme exhibited maximal activity in the presence of 0.75 millimolar Ca(2+), 0.5 millimolar EGTA, and 5 millimolar cellobiose at pH 7.5 and 30°C at 1 milli...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1989
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1061683/ https://ncbi.nlm.nih.gov/pubmed/16666718 |
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