Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA

We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a variety of bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplificati...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Marchesi, Julian R., Sato, Takuichi, Weightman, Andrew J., Martin, Tracey A., Fry, John C., Hiom, Sarah J., Wade, William G.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1998
Soggetti:	Methods
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106123/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9464425/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.2.795-799.1998
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