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Functional Complementation of Nontoxic Mutant Binary Toxins of Bacillus sphaericus 1593M Generated by Site-Directed Mutagenesis

Alanine residues were substituted by site-directed mutagenesis at selected sites of the N- and C-terminal regions of the binary toxin (51- and 42-kDa peptides) of B. sphaericus 1593M, and the mutant toxins were cloned and expressed in Escherichia coli. Bioassays with mosquito larvae, using binary to...

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Detalhes bibliográficos
Main Authors: Shanmugavelu, M., Rajamohan, F., Kathirvel, M., Elangovan, G., Dean, D. H., Jayaraman, Kunthala
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC106113/
https://ncbi.nlm.nih.gov/pubmed/9464418
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