Gene Cloning, Nucleotide Sequencing, and Purification and Characterization of the Low-Specificity l-Threonine Aldolase from Pseudomonas sp. Strain NCIMB 10558

A low-specificity l-threonine aldolase (l-TA) gene from Pseudomonas sp. strain NCIMB 10558 was cloned and sequenced. The gene contains an open reading frame consisting of 1,041 nucleotides corresponding to 346 amino acid residues. The gene was overexpressed in Escherichia coli cells, and the recombi...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Liu, Ji-Quan, Ito, Saeko, Dairi, Tohru, Itoh, Nobuya, Kataoka, Michihiko, Shimizu, Sakayu, Yamada, Hideaki
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1998
Schlagworte:	Enzymology and Protein Engineering
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106081/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9464392/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.2.549-554.1998
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