Optimization of Bacteriocin Release Protein (BRP)-Mediated Protein Release by Escherichia coli: Random Mutagenesis of the pCloDF13-Derived BRP Gene To Uncouple Lethality and Quasi-Lysis from Protein Release

Bacteriocin release proteins (BRPs) can be used for the release of heterologous proteins from the Escherichia coli periplasm into the culture medium. However, high-level expression of BRP causes apparent lysis of the host cells in liquid cultures (quasi-lysis) and inhibition of growth on broth agar...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	van der Wal, Fimme J., Koningstein, G., ten Hagen, C. M., Oudega, Bauke, Luirink, Joen
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1998
Soggetti:	Physiology and Biotechnology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106056/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9464372/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.2.392-398.1998
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