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An improved procedure for utilizing terminal transferase to add homopolymers to the 3' termini of DNA.

Terminal deoxynucleotidyl transferase (E.C.2.7.7.3.1.) from calf thymus was used to add homopolymer tails to duplex DNA with 3' protruding, even, or 3' recessive ends. A gel electrophoresis method was employed to analyze the tail length and the percent of DNA with tails. In all the tailing...

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Sonraí Bibleagrafaíochta
Main Authors: Deng, G, Wu, R
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1981
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1058474/
https://ncbi.nlm.nih.gov/pubmed/6272197
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