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Technical Considerations in the Preparation of Fluorescent-Antibody Conjugates
A comparison was made of (NH(4))(2)SO(4), HCl, ethodin, and ethanol for fractionation of rabbit antiserum prior to conjugation with fluorescein isothiocyanate. Fractionation with the salt was found to be the method of choice from the standpoints of simplicity and recovery of antibody effective in co...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1964
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1058130/ https://ncbi.nlm.nih.gov/pubmed/14201087 |
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