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Generation of Bioluminescent Streptococcus mutans and Its Usage in Rapid Analysis of the Efficacy of Antimicrobial Compounds

The oral bacterium Streptococcus mutans was transformed by electroporation with a shuttle vector (pCSS945) containing insect luciferase gene from a click beetle (Pyrophorus plagiophthalamus) resulting in a bioluminescent phenotype. This S. mutans strain was used in experiments in which light emissio...

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Udgivet i:Antimicrob Agents Chemother
Principais autores: Loimaranta, Vuokko, Tenovuo, Jorma, Koivisto, Leeni, Karp, Matti
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1998
Fag:
Analytical Procedures
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC105708/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9687382/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.42.8.1906
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